Handbook of Molecular and Cellular Methods in Biology and Medicine

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Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

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Biology of Stem Cells and the Molecular Basis of the Stem State

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Biology of Stem Cells and the Molecular Basis of the Stem Cells concentrates upon adult stem cells, particularly on mesenchymal cell populations, which is the author’s area of expertise. The text offers the reader a detailed description of the emergence of stem cell research and the dogmas that were created during the first decades of analysis of stem cell properties, particularly those of hemopoietic stem cells. Biology of Stem Cells and the Molecular Basis of the Stem Cells also introduces the reader to the commonly accepted notions regarding stem cell biology, with an emphasis on an alternative view of stemness, i.e. the stem state. In keeping with the popularity of this topic, Biology of Stem Cells and the Molecular Basis of the Stem Cells addresses the major controversies and points of dispute, among researchers in the stem cell field. Overall, Biology of Stem Cells and the Molecular Basis of the Stem Cells presents a well-rounded dialogue about stem cells as it not only concentrates upon the biological elements of stem cell, but also addresses the controversy and hype currently enveloping this popular subject.

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Applied Cell and Molecular Biology for Engineers

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A Guide to the Fundamentals and Latest Concepts of Molecular and Cell Biology

Bridging the gap between biology and engineering, Applied Cell and Molecular Biology for Engineers uses clear, straightforward language to introduce you to the cutting-edge concepts of molecular and cell biology.

Written by an international team of engineers and life scientists, this vital tool contains “clinical focus boxes” and “applications boxes” in each chapter to link biology and engineering in today’s world. To help grasp complex material quickly and easily, a glossary is provided.

Applied Cell and Molecular Biology for Engineers features:

• Clear descriptions of cell structures and functions
• Detailed coverage of cellular communication
• In-depth information on cellular energy conversion
• Concise facts on information flow across generations
• A succinct guide to the evolution of cells to organisms

Inside This Biomedical Engineering Guide

Biomolecules: • Energetics • Components of the cell • Cell Morphology: • Cell membranes • Cell organelles • Enzyme Kinetics: • Steady-state kinetics • Enzyme inhibition • Cellular Signal Transduction: • Receptor binding • Apoptosis • Energy Conversion: • Cell metabolism • Cell respiration • Cellular Communication: • Direct • Local • Long distance • Cellular Genetics: • DNA and RNA synthesis and repair • Cell Division and Growth: • Cell cycle • Mitosis • Stem cells • Cellular Development: • Germ cells and fertilization • Limb development • From Cells to Organisms: • Cell differentiation • Systems biology

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Text Mining for Biology And Biomedicine

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With the volume of biomedical research growing exponentially worldwide, the demand for information retrieval expertise in the field has never been greater. Hereâ??s the first guide for bioinformatics practitioners that puts the full range of biological text mining tools and techniques at their fingertips in a single dedicated volume. It describes the methods of natural language processing (NLP) and their applications in the biological domain, and spells out the various lexical, terminological, and ontological resources at their disposal â?” and how best to utilize them.

Readers see how terminology management tools like term extraction and term structuring facilitate effective mining, and learn ways to readily identify biomedical named entities and abbreviations. The book explains how to deploy various information extraction methods for biological applications. It helps professionals evaluate and optimize text mining systems, and includes techniques for integrating text mining and data mining efforts to further facilitate biological analyses. Both a critical review of the state of the art and a solution-focused guide packed with â??how toâ? insight, this first-of-its-kind work will prove indispensable for seasoned and first-time bioinformatics researchers alike.

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Molecular Fungal Biology

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Fungi are a diverse group of organisms, studied widely because of their commercial importance in biotechnology, agriculture, and medicine, and because they provide simple model systems for illuminating the eukaryotic mode of life. The advancement of techniques in molecular biology has opened up new areas of mycological research, with important consequences such as the sequencing of the first entire eukaryotic genome. This book focuses on the application of molecular and cell biology to mycology and presents examples of how these approaches are elucidating long-held problems in areas as diverse as animal and plant pathology, protein expression, and evolution. Aimed at advanced undergraduates taking courses in mycology, applied biology, biotechnology, fungal biology and fungal genetics, this textbook will also serve postgraduates needing an introduction to modern fungal research.

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